Journal: BMC Molecular Biology

Article Title: Differential regulation of the rainbow trout ( Oncorhynchus mykiss ) MT-A gene by nuclear factor interleukin-6 and activator protein-1

doi: 10.1186/1471-2199-14-28

Figure Lengend Snippet: Activation of rtMT-A deletion constructs following exposure to PQ. RTH-149 cells were transfected with 0.5 ug of MRE-AP1-NFIL6-pGL3, MRE-AP1-pGL3 and MRE-pGL3 vectors and 0.3 μg of pRL-CMV vector. Following transfection cells were exposed to 10 μM PQ. The activities are given as fold induction. All activities were normalized to the expression level of the pGL3-basic vector. Luciferase activities were analyzed 24 hours post transfection. Results are presented as mean ± SE (n = 4). Statistically significant differences from control levels are indicated by *(p < 0.05); **(p < 0.01).

Article Snippet: The cells were co-transfected with 0.5 μg of enhancer coupled pGL3-basic vector or the empty pGL3-basic vector and 0.3 g of pRL-CMV vector (Renilla Luciferase control reporter vector, Promega, USA), in serum free medium using Lipofectamin 2000 (Invitrogen, USA).

Techniques: Activation Assay, Construct, Transfection, Plasmid Preparation, Expressing, Luciferase

Journal: BMC Molecular Biology

Article Title: Differential regulation of the rainbow trout ( Oncorhynchus mykiss ) MT-A gene by nuclear factor interleukin-6 and activator protein-1

doi: 10.1186/1471-2199-14-28

Figure Lengend Snippet: Activation of rtMT-A deletion constructs following exposure to PQ and PMA. RTH-149 cells were transfected with 0.5 μg of either the NFIL6-pGL3 or the AP1-pGL3 (AP tot ) vectors and 0.3 μg of pRL-CMV vector (Renilla Luciferase control reporter vector). Following transfection cells were exposed to 10 μM PQ (A) or to 162 nM PMA (B) . The activities are given as fold induction. All activities were normalized to the expression level of the pGL3-basic vector. Luciferase activities were analyzed 24 hours post transfection. Results are presented as mean ± SE (n = 4). Statistically significant differences from control levels are indicated by **(p < 0.01).

Article Snippet: The cells were co-transfected with 0.5 μg of enhancer coupled pGL3-basic vector or the empty pGL3-basic vector and 0.3 g of pRL-CMV vector (Renilla Luciferase control reporter vector, Promega, USA), in serum free medium using Lipofectamin 2000 (Invitrogen, USA).

Techniques: Activation Assay, Construct, Transfection, Plasmid Preparation, Luciferase, Expressing